The API Rapid NFT correctly identified 87%, misidentified 5%, gave a low selectivity identification for 8% of the isolates, and in some instances, required up to 48 h of incubation. The API 20E is a valid system for use in the identification of the more commonly occurring members of the family Vibrionaceae and the most accurate and efficient of the three systems tested.
The API 20E correctly identified all the isolates within 24 h. The API Rapid E correctly identified only 77%, misidentified 8%, and failed to identify 2% of the isolates in 4 h. The remaining 13% of the isolates gave a low selectivity identification, with one of the choices being correct.
Comparison of API 20E, API rapid E, and API rapid NFT for identification of members of the family Vibrionaceae. Overman TL, Kessler JF, Seabolt JP. Sixty isolates, from nine species of the family Vibrionaceae, were tested by the API 20E, API Rapid E, and API Rapid NFT systems. Results were compared with those obtained with standard biochemicals.
Keywords: API 20E system, API Rapid NFT system, SBR, CFSTR. The purpose of this study was to test the applicability of the API 20E and API Rapid NFT systems for the identification of some predominant gram-negative and gram-positive bacteria isolated from lab-scale activated sludge treatment systems. In this study, one lab-scale
The API Rapid E gave the same identification as the API 20E for 94.0% (410 of 436) of the isolates, misidentified 3.0% (13 of 436), and gave a correct but low-selectivity answer for the remaining
A total of 223 isolates belonging to motile Aeromonas, A. salmonicida, Vibrio anguillarum, Pasteurella piscicida and Yersinia ruckeri species were tested by the API-20E system and the results compared with those obtained with standard biochemical tube and plate tests. Depending on the species, the API-20E yielded false negative and positive reactions for fermentation of different sugars, lysine decarboxylase …
A study was conducted to compare the API Rapid 20E 4-h system (API System S.A., France; commercially available in the U.S.A. under the name DMS Rapid E …
Comparison of the API 20E and Oxi/Ferm systems in identification of nonfermentative and oxidase-positive fermentative bacteria. J Clin Microbiol. 1979 Feb; 9 (2):220–226.
The API 20E system is a standardized, miniaturized version of conventional procedures for rapid identification of Enterobacteriaceae and other gram-negative bacteria, and the Rapid NFT kit is used for the identification of the gram-negative, non-fermentative bacteria.
The only product to correctly identify at least 90% of V. cholerae strains was the Crystal E/NF, and only three of the six products, the API 20E and both of the Vitek cards, correctly identified more than 90% of the V. parahaemolyticus strains.
Published in: Journal of Clinical Microbiology · 2003Authors: Caroline M Ohara · Evangeline G Sowers · Cheryl A Bopp · Sarah B Duda · Nancy A …About: Vibrio · Vibrio mimicus · Vibrio parahaemolyticus · Vibrio cholerae · Microbiology · Vibri…
The API Rapid E gave the same identification as the API 20E for 94.0% (410 of 436) of the isolates, misidentified 3.0% (13 of 436), and gave a correct but low-selectivity answer for the remaining 3.0% (13 of 436). The API Rapid E is a suitable alternative for the rapid identification of the Enterobacteriaceae.